ID: 4727

Automated nitrogen analysis reduces costs per sample

N-Realizer analytical system from C. Gerhardt increases productivity with valve block from Festo

The world's population is growing and with it the demand for food and animal feed with clearly verifiable ingredients. This includes the protein content. This can be determined by nitrogen analysis – fully automaticated with the new N-Realyzer from C. Gerhardt. The laboratory system ensures high throughput, is low-maintenance and intuitive to operate. Among other things, it owes this to a valve block from Festo, which offers high tightness in gas distribution.

With N-Realyzer, proteins from food, animal feed or fertilizers can be analyzed quickly, easily and reliably using the Dumas method. "In future, the system will also be used to carry out analyses in the growth market of the pharmaceutical industry," explains Nils Müller, Head of Research and Development at C. Gerhardt. The Dumas method offers clear advantages over the wet-chemical Kjeldahl method: it is much faster, requires less preparation and is much safer for laboratory technicians as they do not come into contact with hazardous chemicals.


Nitrogen content determines protein content

From the solid sample to the finished result, the system works through five steps. In the first step, the samples are thrown into the furnace by the autosampler. In the furnace, the samples are being combusted completely at around 1000° C with the help of an oxidation catalyst. The combustion of the samples produces a gas mixture with all possible components – but only the nitrogen content is of interest in order to calculate the protein content.


Step 2 takes place in the copper reactor. There, the nitrogen oxides are reduced to nitrogen pairs and excess oxygen from the combustion is captured. The third step is the removal of water from the gas flow using a two-stage water separation system. After water separation, only the carrier gas, nitrogen and carbon dioxide remain in the gas stream.


High tightness of the valve block for sample and purge gases

"This is where the valve block with the VYKA media-separated solenoid valves from Festo comes into play and helps us to remove the carbon dioxide via our adsorber system," adds Nils Müller. Festo's individually created customer solution distributes sample and purge gas in the CO2 traps. "The valve block meets the strict requirements for tightness and critical operational reliability within the 24-valve configuration of our system," says Nils Müller, pleased with the high tightness of Festo's solution.


The high tightness and low energy consumption of the valve system increases the efficiency of the system and reduces the cooling times of the adsorbers, as the device manages with low operating temperatures overall. The valve block with the VYKA valves therefore contributes to overall productivity. "We were particularly impressed by the commitment and support shown by the Festo experts in their Project Office for the life science market in developing our customer-specific solution," adds Nils Müller. Festo's solution was available ready for installation within four months of the initial contact.


Reduce complexity

Removing CO2 is simple on a small scale, but becomes complex when hundreds of samples are processed every week. "We teamed up with Festo to develop a customized, reliable gas distribution system on the carbon adsorber that makes it much easier to use and automatically clean our adsorbers," explains Nils Müller. After the adsorbers, N-Realyzer has separated all elements of the combustion gas except nitrogen. This means that the nitrogen content can be quantified using a thermal conductivity detector (TCD).


Reduce costs per sample

The patented autosampler and the adsorber system with the valve block from Festo in combination with the sophisticated software offer laboratory users a great deal of flexibility. The working times of the device can be set in the software so that, after appropriate preparation the day before, the device can start work before personnel are present in the laboratory. In addition, hardly any chemicals are used. "These important features significantly reduce the costs per sample," reports Müller.


Whether solid or liquid, with a high or low nitrogen content: N-Realyzer delivers reliable and precise results for up to 100 samples in series for any sample matrix. The patented autosampler prevents samples from being mixed up. Thanks to the high variability in the sample sequence, users can flexibly "insert" urgent samples. 80% solid samples today and 70% liquid or pasty samples tomorrow? That would be no problem for N-Realyzer. Thanks to the two-stage water separation, the water content of aqueous samples is completely removed. The benefit: laboratories can analyze aqueous samples in series without risking the failure of their device.


Sophisticated design

During development, the development team placed great emphasis on improving user and maintenance friendliness. For example, unlike its predecessor model, N-Realyzer is cubic and no longer rectangular. It is also mounted on a turntable. This means that all parts are easily accessible.


N-Realyzer therefore sets standards in terms of availability, service and durability. The quick replacement of all process-relevant components during operation saves working time and ensures smooth operation. "The use of high-quality materials and the carefully considered system design not only ensures precise analysis, but also simple maintenance and servicing. The tool-free sealing concept enables all consumables to be replaced quickly," summarizes Nils Müller, Head of Research and Development.


About C. Gerhardt:

C. Gerhardt GmbH & Co. KG in Königswinter near Bonn has been developing and producing analytical systems and basic products for laboratory work since 1846. In addition to the analytical accuracy of the systems in the laboratories, customers worldwide receive comprehensive applicative support and maintenance services. With three subsidiaries, eight regional offices and numerous international partners, the company is active in over 90% of the world with 100 employees.

费斯托 (Festo)是一家全球性的独立的家族企业,总部位于德国埃斯林根。自成立以来,Festo在工业自动化技术和技术教育方面制定标准,从而为环境、经济和社会的可持续发展做出贡献。公司为超过35个行业的30万家工厂和过程自动化客户提供气动和电驱动自动化技术解决方案,其中生命科学和实验室自动化业务受到越来越多的关注。Festo产品和服务遍布176个国家。2023年,费斯托在全球61个国家的250多个分支机构拥有约20600名员工,实现销售额36.5亿欧元。每年约7%的销售额用于研发。在这家学习型企业,1.5%的销售额用于基础和进一步培训。Festo 教学培训 (Didactic SE) 是全球领先的技术教育和培训供应商,为全球客户提供工业环境中全面的数字化和常规学习解决方案。

© Festo SE & Co. KG
Automated nitrogen analysis to determine protein content: the N-Realyzer from C. Gerhardt increases productivity and significantly reduces costs per sample. .
© Festo SE & Co. KG
Valve block
Valve block with media-separated solenoid valves VYKA from Festo: the high tightness and low energy loss of the valve system increase the efficiency of the machine.
© Festo SE & Co. KG
Nils Müller, C. Gerhardt
Nils Müller, Head of Research and Development at C. Gerhardt